Kesong puti is a Filipino soft white cheese made usually from carabao or cow’s milk using rennet or acid. It is home to different species of beneficial microbes that may potentially display probiotic traits. This study aimed to evaluate the acid and bile resistance, NaCl tolerance, cell surface hydrophobicity (CSH), auto-aggregation, hemolytic, antibacterial, and antibiotic susceptibility characteristics of Lactobacillus plantarum 1074, a rod-shaped, Gram-positive, lactic acid bacterium isolated from kesong puti. The isolate exhibited resistance to pH 3 and 0.5% bile salts. Moreover, it was able to tolerate MRS broth with 8% NaCl. In terms of cell surface properties, the isolate displayed strong auto-aggregation activity. It also showed a higher affinity to xylene indicating cell surface hydrophobicity. When grown to blood agar, the isolate demonstrated gamma (γ)-hemolytic activity suggesting its non-hemolytic nature. In terms of inhibitory activity, it exhibited strong antibacterial activity against Escherichia coli (laboratory isolate). The antibiotic susceptibility pattern of L. plantarum 1074 displayed resistance to cefaclor and kanamycin. However, it expressed sensitivity to amoxicillin, ampicillin, augmentin, penicillin, streptomycin, chloramphenicol, clindamycin, erythromycin, and tetracycline. The findings of this study revealed the promising potential of Lactobacillus plantarum 1074 as a probiotic strain as it exhibited desirable attributes in vitro.